Low Cost Microfluidic Device for Assaying Blood Glucose. ![]() KEYWORDS: Capillary Driven Microfluidic Device Point-of-Care Diagnostics Silica Gelĭownload this article as: Copy the following to cite this article: Microfluidic device developed is cheap and suitable for low cost setting areas. The developed microfluidic device do not require (i) pump or device to propel the fluid (ii) any type of special mesh or sieve to separate the serum from the blood. Determination of the glucose in the blood is carried out on a desktop scanner. The colour of the silica beads immobilized with enzymes and dye changes from white to orange when comes in contact with glucose in serum. The separated serum then comes in contact with the silica gel immobilized with enzymes and dye. Serum separation started within 30 seconds and subsequently within 2 min., serum successfully separates from blood by pure silica gel. Without applying any external power, blood moves through the silica packed region by gravitational pull and capillary action of silica gel. One drop of blood is poured at the inlet of microfluidic device. Whereas, third and the top most layer is filled with untreated silica gel. Second layer from the bottom i.e the layer above PVC layer is filled with silica gel immobilized with horse radish peroxidase (HRP), glucose oxidase (GOD) and o- dianisidine (dye). ![]() Bottom of the refill is filled with the polymer polyvinyl chloride (PVC). Refill is filled with three layers of polymer. Microfluidic system is developed by using discarded pen refills. College, Badalpur, Gautam Buddha Nagar (U.P.) – 203207, India.ģNew Idea Lab, C-202, Ramkrishnan Cooperative Group Housing Society, Plot-12, Sector 23, Dwarka, New Delhi 110077, India.ĪBSTRACT: Herein, gravitational force based low cost colorimetric microfluidic device is developed for diagnostic purpose. College, Badalpur, Gautam Buddha Nagar (U.P.) – 203207, India.ĢDepartment of Zoology, Km. Sharma 2 and Pradip Nahar 3ġWomen Scientist (WOSB, DST), Department of Zoology, Km. Low Cost Microfluidic Device for Assaying Blood GlucoseĪzmi Naqvi* 1, Dinesh C.
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